Differences between Vampire: The Masquerade and Vampire: The Requiem

Vampire: The Masquerade went through Revisions before ending an Vampire The Requirem replacing it. In that time, the game acquired a lot of baggage that has since been swept clean. This article summarizes those revisions.

Basic Mechanics
Vampires no longer have a Generation, Blood Potency fulfills some of the same functions, but it is a far more variable attribute.

Embracing and Ghouling are more expensive in VtR, costing a Willpower point (permanent for embrace, temporary for ghoul) every time the deed is done. This limits Ghoul Abuse and a variety of other tactics used in VtM, especially Mass Embrace.

Clans
There are fewer clans, and all VtR vampires are members of a clan. There are no Caitiff.

Bloodlines are now formally defined, with a mechanism for joining them.

Background
Vampires are ahistorical in VtR; unlike VtM with a definite beginning and Vampires who might conceivably remember it, the Torpor mechanism in VtR ensures that the true history of Vampirism is largely unknowable.

VtR had a Camarilla, but it ended sometime around the time of Rome.